Chemical indicator results are obtained immediately following the sterilization cycle and therefore can provide more timely information about the sterilization cycle than a spore test.Ī chemical indicator should be used inside every package to verify that the sterilizing agent has penetrated the package and reached the instruments inside. Examples include chemical indicator tapes, strips, or tabs and special markings on packaging materials. Since these parameters can be observed during the sterilization cycle, this might be the first indication of a problem.Ĭhemical monitoring uses sensitive chemicals that change color when exposed to high temperatures or combinations of time and temperature. Mechanical monitoring involves checking the sterilizer gauges, computer displays, or printouts, and documenting in your sterilization records that pressure, temperature, and exposure time have reached the levels recommended by the sterilizer manufacturer. Mechanical and chemical monitoring should be done for every sterilizer load. Mechanical and chemical indicators do not guarantee sterilization however, they help detect procedural errors (e.g., overloaded sterilizer, incorrect packaging) and equipment malfunctions. However, because spore tests are only done weekly and the results are usually not obtained immediately, mechanical and chemical monitoring should also be done. Biological indicators, or spore tests, are the most accepted means of monitoring sterilization because they assess the sterilization process directly by killing known highly resistant microorganisms (e.g., Geobacillus or Bacillus species). Sterilization procedures should be monitored using biological, mechanical, and chemical indicators.
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